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rabbit anti 8 ohdg  (Bioss)


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    Structured Review

    Bioss rabbit anti 8 ohdg
    Rabbit Anti 8 Ohdg, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+8+ohdg+antibody/8-OHdG+Polyclonal+Antibody/pm42089455-111-6-8
    Average 96 stars, based on 218 article reviews
    rabbit anti 8 ohdg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunostaining:

    Article Title: PCPE-1 promotes cardiac fibrosis with aging and obesity.
    Article Snippet: Histological analyses and immunostaining Samples of brown adipose tissue (BAT) and heart (left ventricle [LV]) were harvested, fixed overnight in 10% formalin, embedded in paraffin, and sectioned for immunofluorescence, hematoxylin-eosin (HE) (Hematoxylin and Eosin Stain Kit, ScyTek Laboratories, Inc., HAE-1) or Masson's trichrome stain (Trichrome Stain Kit, ScyTek Laboratories, Inc., TRM-1)(38), and photographed with a Biorevo (Keyence Co., Osaka, Japan). .. For immunostaining, BAT deparaffinized sections were retrieved with citrate buffer, brown adipocytes were fixed with 4% Paraformaldehyde (4% PFA, Thermo Scientific, J61899.AK), and incubated with anti-8-OHdG antibody (Bioss, bs-1278R, Lot: BD1006918425), and anti-phospho-γH2AX (Ser139) antibody (Cell Signaling, 9718, Lot:21, Clone #20E3) at 1:100 dilution. .. Anti-rabbit, Cy5 conjugate (Abcam, ab6564) at 1:200 dilution, was used as a secondary antibody.

    Incubation:

    Article Title: PCPE-1 promotes cardiac fibrosis with aging and obesity.
    Article Snippet: Histological analyses and immunostaining Samples of brown adipose tissue (BAT) and heart (left ventricle [LV]) were harvested, fixed overnight in 10% formalin, embedded in paraffin, and sectioned for immunofluorescence, hematoxylin-eosin (HE) (Hematoxylin and Eosin Stain Kit, ScyTek Laboratories, Inc., HAE-1) or Masson's trichrome stain (Trichrome Stain Kit, ScyTek Laboratories, Inc., TRM-1)(38), and photographed with a Biorevo (Keyence Co., Osaka, Japan). .. For immunostaining, BAT deparaffinized sections were retrieved with citrate buffer, brown adipocytes were fixed with 4% Paraformaldehyde (4% PFA, Thermo Scientific, J61899.AK), and incubated with anti-8-OHdG antibody (Bioss, bs-1278R, Lot: BD1006918425), and anti-phospho-γH2AX (Ser139) antibody (Cell Signaling, 9718, Lot:21, Clone #20E3) at 1:100 dilution. .. Anti-rabbit, Cy5 conjugate (Abcam, ab6564) at 1:200 dilution, was used as a secondary antibody.

    Article Title: Diosmetin improves myocardial ischemia/reperfusion injury via activation of the SIRT1/NRF2 axis.
    Article Snippet: Purpose: Myocardial ischemia/reperfusion (I/R) injury is a potential complication associated with ischemic heart disease after recanalization.. Diosmetin (Dios) is a natural flavonoid found in citrus species with therapeutic effects on several cardiovascular conditions.. This study aims to explore the protective effects and mechanisms of Dios on myocardial I/R injury.

    Article Title: Abnormal Ribosome Biogenesis Partly Induced p53-Dependent Aortic Medial Smooth Muscle Cell Apoptosis and Oxidative Stress
    Article Snippet: .. The sections were processed as above and incubated with primary anti-8-OHdG antibody (1 : 200, Bioss, Cat# bs-1278R) and anti- α -SMA antibody (1 : 200, Servicebio, Cat# GB13044). .. The slides were then incubated with a secondary antibody conjugated with a fluorescent label (Cy3-conjugated goat anti-rabbit IgG (H+L) and FITC-conjugated goat anti-mouse IgG (H+L)) (1 : 200, Servicebio, Cat# GB21303 and GB22301) for 1 hour at room temperature and the cell nuclei counterstained with DAPI.

    Article Title: The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus
    Article Snippet: .. For 8-OHdG labeling, samples were immunostained in a Leica BOND-Max instrument (Leica Biosystems, Concord, ON, Canada) using the BOND Epitope Retrieval Solution 1 (#AR9961, Leica Biosystems), and incubated with a primary rabbit anti–8-OHdG antibody (#bs-1278R, Bioss, Atlanta, GA) for 1 hr (dilution 1:500 i.e. 2 μg/ml), followed by detection with a Bond Polymer Refine Detection kit (#DS9800, Leica Biosystems), containing a peroxide block, a polymer reagent, DAB (3,3'-Diaminobenzidine) chromogen, and a hematoxylin counterstain, as per the manufacturer’s guidelines. .. Slides were scanned at 20 x using an Olympus VS-120 scanner (Evident, Tokyo, Japan).

    Immunofluorescence:

    Article Title: Diosmetin improves myocardial ischemia/reperfusion injury via activation of the SIRT1/NRF2 axis.
    Article Snippet: Purpose: Myocardial ischemia/reperfusion (I/R) injury is a potential complication associated with ischemic heart disease after recanalization.. Diosmetin (Dios) is a natural flavonoid found in citrus species with therapeutic effects on several cardiovascular conditions.. This study aims to explore the protective effects and mechanisms of Dios on myocardial I/R injury.

    Staining:

    Article Title: Diosmetin improves myocardial ischemia/reperfusion injury via activation of the SIRT1/NRF2 axis.
    Article Snippet: Purpose: Myocardial ischemia/reperfusion (I/R) injury is a potential complication associated with ischemic heart disease after recanalization.. Diosmetin (Dios) is a natural flavonoid found in citrus species with therapeutic effects on several cardiovascular conditions.. This study aims to explore the protective effects and mechanisms of Dios on myocardial I/R injury.

    Article Title: Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma
    Article Snippet: .. After blocking endogenous peroxidase activity and nonspecific binding sites, the sections were sequentially stained with an anti-TXN antibody (1:400; Abways, CY6679)/TSA 520, an anti-8-OHdG antibody (1:200; Bioss, bs-1278R)/TSA 570, and an anti-HIF-1α antibody (1:200; Abways, CY5197)/TSA 690. ..

    Blocking Assay:

    Article Title: Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma
    Article Snippet: .. After blocking endogenous peroxidase activity and nonspecific binding sites, the sections were sequentially stained with an anti-TXN antibody (1:400; Abways, CY6679)/TSA 520, an anti-8-OHdG antibody (1:200; Bioss, bs-1278R)/TSA 570, and an anti-HIF-1α antibody (1:200; Abways, CY5197)/TSA 690. ..

    Article Title: The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus
    Article Snippet: .. For 8-OHdG labeling, samples were immunostained in a Leica BOND-Max instrument (Leica Biosystems, Concord, ON, Canada) using the BOND Epitope Retrieval Solution 1 (#AR9961, Leica Biosystems), and incubated with a primary rabbit anti–8-OHdG antibody (#bs-1278R, Bioss, Atlanta, GA) for 1 hr (dilution 1:500 i.e. 2 μg/ml), followed by detection with a Bond Polymer Refine Detection kit (#DS9800, Leica Biosystems), containing a peroxide block, a polymer reagent, DAB (3,3'-Diaminobenzidine) chromogen, and a hematoxylin counterstain, as per the manufacturer’s guidelines. .. Slides were scanned at 20 x using an Olympus VS-120 scanner (Evident, Tokyo, Japan).

    Activity Assay:

    Article Title: Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma
    Article Snippet: .. After blocking endogenous peroxidase activity and nonspecific binding sites, the sections were sequentially stained with an anti-TXN antibody (1:400; Abways, CY6679)/TSA 520, an anti-8-OHdG antibody (1:200; Bioss, bs-1278R)/TSA 570, and an anti-HIF-1α antibody (1:200; Abways, CY5197)/TSA 690. ..

    Binding Assay:

    Article Title: Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma
    Article Snippet: .. After blocking endogenous peroxidase activity and nonspecific binding sites, the sections were sequentially stained with an anti-TXN antibody (1:400; Abways, CY6679)/TSA 520, an anti-8-OHdG antibody (1:200; Bioss, bs-1278R)/TSA 570, and an anti-HIF-1α antibody (1:200; Abways, CY5197)/TSA 690. ..

    Labeling:

    Article Title: The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus
    Article Snippet: .. For 8-OHdG labeling, samples were immunostained in a Leica BOND-Max instrument (Leica Biosystems, Concord, ON, Canada) using the BOND Epitope Retrieval Solution 1 (#AR9961, Leica Biosystems), and incubated with a primary rabbit anti–8-OHdG antibody (#bs-1278R, Bioss, Atlanta, GA) for 1 hr (dilution 1:500 i.e. 2 μg/ml), followed by detection with a Bond Polymer Refine Detection kit (#DS9800, Leica Biosystems), containing a peroxide block, a polymer reagent, DAB (3,3'-Diaminobenzidine) chromogen, and a hematoxylin counterstain, as per the manufacturer’s guidelines. .. Slides were scanned at 20 x using an Olympus VS-120 scanner (Evident, Tokyo, Japan).

    Polymer:

    Article Title: The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus
    Article Snippet: .. For 8-OHdG labeling, samples were immunostained in a Leica BOND-Max instrument (Leica Biosystems, Concord, ON, Canada) using the BOND Epitope Retrieval Solution 1 (#AR9961, Leica Biosystems), and incubated with a primary rabbit anti–8-OHdG antibody (#bs-1278R, Bioss, Atlanta, GA) for 1 hr (dilution 1:500 i.e. 2 μg/ml), followed by detection with a Bond Polymer Refine Detection kit (#DS9800, Leica Biosystems), containing a peroxide block, a polymer reagent, DAB (3,3'-Diaminobenzidine) chromogen, and a hematoxylin counterstain, as per the manufacturer’s guidelines. .. Slides were scanned at 20 x using an Olympus VS-120 scanner (Evident, Tokyo, Japan).



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